Interaction between odontoblast and bio-calcium phosphate cement reinforced with chitosan
전병도, 김성원, 이성탁, 김태훈, 이정한, 김규천, 김욱규,
소속 상세정보
전병도 ( Chun Byung-Do ) - 부산대학교 치의학전문대학원 구강악안면외과학교실
김성원 ( Kim Sung-Won ) - 부산대학교 치의학전문대학원 구강악안면외과학교실
이성탁 ( Lee Sung-Tak ) - 부산대학교 치의학전문대학원 구강악안면외과학교실
김태훈 ( Kim Tae-Hoon ) - 부산대학교 치의학전문대학원 구강악안면외과학교실
이정한 ( Lee Jung-Han ) - 부산대학교 치의학전문대학원 구강악안면외과학교실
김규천 ( Kim Gyoo-Cheon ) - 부산대학교 치의학전문대학원 구강악안면외과학교실
김욱규 ( Kim Uk-Kyu ) - 부산대학교 치의학전문대학원 구강악안면외과학교실
KMID : 0355620110370050415
Abstract
Purpose:Calcium phosphate cement (CPC) is one of many useful materials for restoring tooth defects, periodontium and maxillofacial area. Chitosan is a biodegradable material that has been shown to promote the growth and differentiation of osteoblasts in culture. This study examined the interaction between odontoblasts and bio-calcium phosphate cement reinforced with chitosan.
Materials and Methods : 5×103 odontoblastic cells were seeded into each well. Various concentrations of bio-calcium phosphate cement reinforced with chitosan (10, 20, 50, 100, 200, 500 μg/ml, 1, 2, 4 mg/ml) were diluted and added to the wells. The well was incubated for 24 h, 48 h and 72 h. After incubation, the number of cells was assessed to determine the cell viability. A cytokinesis-block micronucleus assay and chromosomal aberration test were carried out to estimate the extent of chromosomal abnormalities. Microscopic photographs and RT-PCR were performed to examine the adhesion potential of bio-calcium phosphate cement reinforced with chitosan.
Results:Bio-CPC-reinforced chitosan did not show significant cytotoxicity. The number of damaged chromosomes in the cells treated with Bio- CPC-reinforced chitosan was similar to that in the control cells. There was no significant increase in the number of chromosomal aberrations in the Bio-CPC reinforced chitosan exposed cells. Microscopic photographs and RT-PCR confirmed the adhesive potential of bio-CPC reinforced chitosan to odontoblasts.
Conclusion:Bio-CPC-reinforced chitosan did not affect the odontoblastic cell viability, and had no significant cytotoxic effect. Bio-CPC-reinforced chitosan showed adhesive potential to odontoblasts. These results are expected form the basis of future studies on the effectiveness of dental restorative materials in Bio-CPC reinforced with chitosan.
키워드
Calcium phosphate cement; Chitosan; Odontoblast
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